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This volume provides all the essential protocols that are currently used to study the immune synapse. Chapters in The Immune Synapse: Methods and Protocols cover methods for the study of the dynamics of immune synapse assembly, traffic at the immune synapse, new high resolution imaging, biophysical and computational methods for the study of the immune synapse, effector immune synapses, B cell, NK and mast cell immune synapses, and immune interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Immune Synapse: Methods and Protocols will be of interest to immunologists
This volume provides all the essential protocols that are currently used to study the immune synapse. Chapters in The Immune Synapse: Methods and Protocols cover methods for the study of the dynamics of immune synapse assembly, traffic at the immune synapse, new high resolution imaging, biophysical
This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful
In this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis
This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile
This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and
This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions
Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known
This volume provides state-of-the-art techniques for studying various aspects of cholesterol homeostasis, including its uptake, synthesis and efflux from the cell, as well as its trafficking within the cell. Chapters also cover techniques for studying the regulation of cholesterol homeostasis at
Preface... Table of Contents...Contributing Authors... Part I Overview1. Introduction: History of SH2 Domains and Their ApplicationsBernard A. Liu and Kazuya Machida 2. What Have We Learned from SH2 Domains?Bruce J. Mayer Part II Computational Approaches 3. Hidden Markov Models for Protein Domain
The second edition of this volume focuses on applied bioinformatics with specific applications to crops and model plants. Plant Bioinformatics: Methods and Protocols is aimed at plant biologists who have an interest in, or requirement for, accessing and manipulating huge amounts of data being
This volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with
As two of the leading causes of death worldwide, heart disease and stroke represent a clear target for genomic research aimed at deciphering the genes and cellular pathways that underlie cardiovascular disease and creating improved therapies. In Cardiovascular Genomics: Methods and Protocols,
The immune system is central to human health and the focus of much medical research. Growing understanding of the immune system, and especially the creation of immune memory (long lasting protection), which can be harnessed in the design of vaccines, have been major breakthroughs in medicine. In
This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via
Demographic Methods and Concepts presents the methods most commonly needed to work with statistical materials on population at national, regional, and local levels. Unlike other texts in the field, this book uses clear non-technical language throughout to make demographic techniques accessible to a
Take an in-depth tour of core Internet protocols and learn how they work together to move data packets from one network to another. With this updated edition, you'll dive into the aspects of each protocol, including operation basics and security risks, and learn the function of network hardware
Wildlife Forensics: Methods and Applications provides an accessible and practical approach to the key areas involved in this developing subject. The book contains case studies throughout the text that take the reader from the field, to the lab analysis to the court room, giving a complete insight
This monograph provides both a unified account of the development of models and methods for the problem of estimating equilibrium traffic flows in urban areas and a survey of the scope and limitations of present traffic models. The development is described and analyzed by the use of the powerful
The seventh edition of Research Methods and Statistics in Psychology provides students with the most readable and comprehensive survey of research methods, statistical concepts and procedures in psychology today. Assuming no prior knowledge, this bestselling text takes you through every stage of
This is a thorough exploration of the models and methods of financial econometrics by one of the world's leading financial econometricians and is for students in economics, finance, statistics, mathematics, and engineering who are interested in financial applications. Based on courses taught around
Heal yourself from the inside out | Our immune systems can no longer be taken for granted. Current trends in public healthcare are disturbing: our increased air travel allows newly mutated bacteria and viruses to spread across the globe, antibiotic-resistant strains of bacteria outstrip the new drugs
How have theatre and performance research methods and methodologies engaged the expanding diversity of performing arts practices? How can students best combine performance/theatre research approaches in their projects? This book's 29 contributors provide hands-on answers to such