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Engineering Multicellular Systems: Methods and Protocols, focuses on laboratory procedures used in recent efforts for constructing synthetic multicellular systems and their applications. In particular, constructing multicellular systems to form various microbial ecosystems has been extensively explored to examine evolution and interactions of microbial ecosystems, while co-cultures have emerged as an efficient tool to produce some complex chemical molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Engineering Multicellular Systems: Methods and Protocols provide a comprehensive laboratory protocol reference
Engineering Multicellular Systems: Methods and Protocols, focuses on laboratory procedures used in recent efforts for constructing synthetic multicellular systems and their applications. In particular, constructing multicellular systems to form various microbial ecosystems has been extensively
This Methods in Molecular Biology(TM) volume offers detailed protocols, with theoretical background, and step-by-step instructions on implementing synchronization, as well as current techniques for the enhanced study of regulatory mechanisms in cell cycle
This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and
This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical
This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful
This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions
This volume details fast-moving research while providing in-depth descriptions of methods and analytical approaches that are helping to understand the genome and how it is related to complex diseases. Chapters guide the reader through common and rare variation, gene-gene and gene-environment
This book presents a compilation of methods that detail improved protein and peptide sample preparation and identification. Chapters guide readers through methods for depletion of myofibril-associated proteins, peptide sample preparation in urinary proteomics, purification of targeted proteins from
Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known
Understanding Hydraulics: The Design, Analysis, and Engineering of Hydraulic Systems Fundamentals of Hydraulic Engineering Systems bridges the gap between fundamental principles and techniques applied to the design and analysis of hydraulic engineering systems. An extension of fluid mechanics,
This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile
The new edition of the definitive reference to trading systems--expanded and thoroughly updated. Professional and individual traders haverelied on Trading Systems and Methods for over three decades. Acclaimed trading systems expert Perry Kaufman provides complete, authoritative information on
This volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with
As two of the leading causes of death worldwide, heart disease and stroke represent a clear target for genomic research aimed at deciphering the genes and cellular pathways that underlie cardiovascular disease and creating improved therapies. In Cardiovascular Genomics: Methods and Protocols,
The Latest Methods for Preventing and Controlling Corrosion in All Types of Materials and Applications Now you can turn to Corrosion Engineering for expert coverage of the theory and current practices you need to understand water, atmospheric, and high-temperature corrosion processes. This
The second edition of this volume focuses on applied bioinformatics with specific applications to crops and model plants. Plant Bioinformatics: Methods and Protocols is aimed at plant biologists who have an interest in, or requirement for, accessing and manipulating huge amounts of data being
CDMA Techniques for Third Generation Mobile Systems presents advanced techniques for analyzing and developing third generation mobile telecommunication systems. Coverage includes analysis of CDMA-based systems, multi-user receivers, Turbo coding for mobile radio applications, spatial and temporal
This book presents methods useful for analyzing and understanding large data sets that are dynamically dependent. The book will begin with examples of multivariate dependent data and tools for presenting descriptive statistics of such data. It then introduces some useful statistical methods for
This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via
This volume covers several aspects of rational drug design, such as synthesis of novel bioactive
A graduate-level textbook that presents a unified mathematical framework for modeling and analyzing cyber-physical systems, with a strong focus on verification. Verification aims to establish whether a system meets a set of requirements. For such cyber-physical systems as driverless cars,
If engineering is the art and science of technical problem solving, systems architecting happens when you don't yet know what the problem is. The third edition of a highly respected bestseller, The Art of Systems Architecting provides in-depth coverage of the least understood part of systems